Sorting Reservation Information

Sorting Reservation Information

Please fill out the following form with details specific to your sorting needs. *DO NOT REQUEST TO SCHEDULE A SORT IF YOU DO NOT HAVE AN ILABS ACCOUNT*

Before You Sort Guide

Please refer to the PDF below for any sorting considerations.

BEFORE YOUR SORT GUIDE

First Last Name*

MUST ENTER FIRST AND LAST NAME

Email Address*

Cell Phone Number*

Please enter your cell phone number so we can contact you with any questions. (No international cell phone numbers please).

Phone

Lab*

If your lab is not listed, please select "Other"

Select

Other Lab*

You may manually type in your lab in this section

Select or enter value

Is your lab located within DFCI or externally?*

Select

Institute Name

Select

Company Name

Project Code*

Please enter the project code with any descriptions (if applicable) that you are using for this reservation.

Purchase Order Number*

Please enter your Purchase Order number you wish to use for this reservation. Please make sure the Purchase Order number is written down correctly. Reservations will not be scheduled without correct Purchase Order numbers.

Lenti Virus

Please indicate here if your cells have been infected with Lentivirus.

Human Primary Cells

Please indicate here if your cells are from human primary cell lines.

Sort Duration (Hours)*

Please enter how much time you will need to sort your samples (Enter just the number of hours, i.e 1.0, 2.0, 2.5, etc)

Select

Nozzle*

Select

Type of Collection Vessel*

Select or enter value

Self-Sort

TRAINED USERS ONLY: Is this request for a self-sort on the SONY MA-900?

SONY Reservation Time

Please list the preferred instrument (if any), DATE, and TIME.

Check iLAB schedule for available times.

If unsure leave this field blank and the core will contact you.

Instrument Considerations*

There are certain instrument limitations that must be considered. If your assay meets any of the criteria below, plesae let the core staff know when scheduling your sort over the phone.

UV Fluorophores → JF Symphony UV ONLY

6-way Sorting → JF Symphony UV & M Symphony ONLY

BV570/Pac Orange/LssmOrange → JF Aria & M Aria ONLY

Ratio Sort → JF Aria & M Aria ONLY

***Sony MA900 Sorters are Co-linear systems, larger assays may not be compatible, make sure to confirm with core***

Please check below to confirm you have taken these considerations into account.

Existing Template?*

Will the core be using an existing template from a prior sort?

Select or enter value

Template Name*

Refer to previous sort report PDFs for the template name. Must be exact match.

Fluorophore Selection

Below, please select your filters/fluorophores that will be used for your experiement.

If your fluorophore is not listed in the dropdown, please select the first option in the corresponding filter.

Ex: If you are using mScarlet, please select PE in the Yellow/Green 582/15 dropdown.

Fluorochrome Selection-UltraViolet 379/28*

Please select the fluorochrome being used for this filter. If not using this filter, please select none.

Select or enter value

Fluorochrome Selection-UltraViolet 450/50*

Please select the fluorochrome being used for this filter. If not using this filter, please select none.

Select or enter value

Fluorochrome Selection-UltraViolet 515/30*

Please select the fluorochrome being used for this filter. If not using this filter, please select none.

Select or enter value

Fluorochrome Selection-UltraViolet 586/15*

Please select the fluorochrome being used for this filter. If not using this filter, please select none.

Select or enter value

Fluorochrome Selection-UltraViolet 610/20*

Please select the fluorochrome being used for this filter. If not using this filter, please select none.

Select or enter value

Fluorochrome Selection-UltraViolet 740/35*

Please select the fluorochrome being used for this filter. If not using this filter, please select none.

Select or enter value

Fluorochrome Selection-UltraViolet 820/60*

Please select the fluorochrome being used for this filter. If not using this filter, please select none.

Select or enter value

Fluorochrome Selection-Violet 450/50*

Please select the fluorochrome being used for this filter. If not using this filter, please select none.

Select or enter value

Fluorochrome Selection-Violet 525/50*

Please select the fluorochrome being used for this filter. If not using this filter, please select none.

Select or enter value

Fluorochrome Selection-Violet 582/15*

Please select the fluorochrome being used for this filter. If not using this filter, please select none.

Select or enter value

Fluorochrome Selection-Violet 610/20*

Please select the fluorochrome being used for this filter. If not using this filter, please select none.

Select or enter value

Fluorochrome Selection-Violet 660/20*

Please select the fluorochrome being used for this filter. If not using this filter, please select none.

Select or enter value

Fluorochrome Selection-Violet 710/20*

Please select the fluorochrome being used for this filter. If not using this filter, please select none.

Select or enter value

Fluorochrome Selection-Violet 780/60*

Please select the fluorochrome being used for this filter. If not using this filter, please select none.

Select or enter value

Fluorochrome Selection-Blue 530/30*

Please select the fluorochrome being used for this filter. If not using this filter, please select none.

Select or enter value

Fluorochrome Selection-Blue 710/50*

Please select the fluorochrome being used for this filter. If not using this filter, please select none.

Select or enter value

Fluorochrome Selection-Yellow/Green 582/15*

Please select the fluorochrome being used for this filter. If not using this filter, please select none.

Select or enter value

Fluorochrome Selection-Yellow/Green 610/20*

Please select the fluorochrome being used for this filter. If not using this filter, please select none.

Select or enter value

Fluorochrome Selection-Yellow/Green 660/20*

Please select the fluorochrome being used for this filter. If not using this filter, please select none.

Select or enter value

Fluorochrome Selection-Yellow/Green 780/60*

Please select the fluorochrome being used for this filter. If not using this filter, please select none.

Select or enter value

Fluorochrome Selection-Red 660/20*

Please select the fluorochrome being used for this filter. If not using this filter, please select none.

Select or enter value

Fluorochrome Selection-Red 710/50*

Please select the fluorochrome being used for this filter. If not using this filter, please select none.

Select or enter value

Fluorochrome Selection-Red 780/60*

Please select the fluorochrome being used for this filter. If not using this filter, please select none.

Select or enter value

NOTE

Any additional information you would like to provide to the core

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