TDAAC Histology Service Submission Form

TDAAC Histology Service

Submission Form

Service Fee link

Any questions please email TDAACHisto@vcu.edu

Date of Submission/Start Date*

Please upload an excel file listing sample ID# and organ type

save the excel file using the following format: PI last name_order date, e.g., Koblinski_09082022

Drag and drop files here or

What is the species and the type of tissue you are submitting?*

e.g., mouse with mouse tumor, mouse with human tumor xenograft, human lung, mouse liver, etc

How would you like your sample grossed?*

i.e. special instructions for grossing

When were your samples fixed?*

Number of tissues to be grossed?*

Other fixative used*

Other fixative/buffer samples stored in?*

Are your samples already in cassettes?*

yes

How many samples need to be processed & embedded?*

Any special processing requests? If yes, describe*

Please describe how you want the samples embedded.*

(specific orientation requests)

Are you submitting samples already in blocks?*

yes

Number of paraffin blocks submitting?*

Are you submitting slides already sectioned?*

yes

How many sectioned slides are you submitting?*

How many unstained slides per sample do you want?*

Please only indicate the number of unstained slides you would like. These are ones you would take back to your lab with no staining. If you are also doing H&E, IHC/IF, mIF, or mISH the slide count sectioned will be included in those sections below.

How many sections per slide do you want?*

(additional charge for greater than 2 secitons/slide)

How many H&E slides stained per sample?*

Is there a specific slide you want stained for H&E*

e.g. the 3rd slide cut out of 6 (1st slides is the first sections cut)

Is there specific slide to be stained for IHC/IF*

e.g. the 3rd slide cut out of 6 (1st slides is the first sections cut)

Do you want the staining to be IHC or IF?*

IHC

What antibody(ies) do you want used for IHC or IF?*

Will your staining need to be optimized?*

Please see the list of optimized antibodies here. If the antibody is not listed please select yes, optimization is necessary.

yes

Will your lab provide the antibody for IHC/IF?*

yes

Any specific instructions for IHC/IF?

Is there a specific slide to be stained for mIF?*

e.g. the 3rd slide cut out of 6 (1st slides is the first sections cut)

What mIF panel would you like?*

Human immune panel -1 (CD3, CD8, FOXP3, granzyme B, CD45R, Pan-Cytokeratin)

Human immune panel -2 (CD3, CD8, FOXP3, granzyme B, CD45R, PDL1, Pan-cytokeratin)

Human immune panel-3 (CD8, CD68, PDL, PDL1, FOXP3, Pan-cytokeratin)

Mouse immune panel-1 (CD4, CD8, FOXP3, CD11b, GR1, pan-cytokeratin)

Mouse immune panel-2 ( F4/80, CD80, Arginase, GR1, CD11b, pan-cytokeratin)

Other- need consultation

Is there a specific slide to be stained for ISH?*

e.g. the 3rd slide cut out of 6 (1st slides is the first sections cut)

What probes will you use for ISH?*

Please include which fluorophore you would like paired with each probe.

What is the total number of slides that will be submitted for imaging?*

What type of imagining would you like?*

Select or enter value

What magnification would you like?*

Note: Higher magnification creates larger files

10X

20X

40X

Is the slide a TMA?*

yes

What fluorophores did you use for your IF?*

Do you want to select regions of interest to perform digital quantification??

Select

How many of each consumables do you need?*

Who do you want to run your samples for the HemaVet?*

please remember to sign up using the Force system

Self/lab running machine

TDAAC running machine

How many samples do you have to run on the Hemavet?*

Please provide any other comments or detailed instrucitons

Send me a copy of my responses