Duke Regional Biocontainment Laboratory (RBL)

 
 
 
 

Services Offered- Duke RBL

RBL Immunology Unit

Commercial Luminex

Luminex using beads conjugated to antigen in-house

Luminex bead labeling

Luminex instrument use (read only)

Commercial ELISA

End point titer ELISA

sjTREC quantification (signal joint T-cell receptor excision circle)

Quantigene assay

MACS cell enrichment

MSD assay

ELISpot instrument use (read only)


RBL Pathogens Unit

Microneutralization assay

Hemagglutination inhibition assay (HAI)

Plaque assay

Focus forming assay

TCID50 assay

SARS2 viral load (qRT-PCR- N1 gene)

SARS2 viral load (qRT-PCR- N/E subgenomic)

SARS2 anti-viral testing

SARS2 cell culture infection

Virus propagation/QC


RBL Animal Models Unit

Immunogenicity/treatment

Immunogenicity/treatment + pathogen challenge

Pathogen challenge

IVIS imaging


Other

NA-ELLA

ADCC/ADCP/ADNP (human, mouse)

Fc-gamma binding

Routine surface phenotyping

Phenotyping with in vitro stimulation and intracellular staining

Cell sorting

Cell separations

Biomarker/Metabolite Profiling

BCR/TCR Repertoire