Duke Regional Biocontainment Laboratory (RBL)

Services Offered- Duke RBL

RBL Immunology Unit

Commercial Luminex

Luminex using beads conjugated to antigen in-house

Luminex bead labeling

Luminex instrument use (read only)

Commercial ELISA

End point titer ELISA

sjTREC quantification (signal joint T-cell receptor excision circle)

Quantigene assay

MACS cell enrichment

MSD assay

ELISpot instrument use (read only)


RBL Pathogens Unit

Microneutralization assay

Hemagglutination inhibition assay (HAI)

Plaque assay

Focus forming assay

TCID50 assay

SARS2 viral load (qRT-PCR- N1 gene)

SARS2 viral load (qRT-PCR- N/E subgenomic)

SARS2 anti-viral testing

SARS2 cell culture infection

Virus propagation/QC


RBL Animal Models Unit

Immunogenicity/treatment

Immunogenicity/treatment + pathogen challenge

Pathogen challenge

IVIS imaging


Other

NA-ELLA

ADCC/ADCP/ADNP (human, mouse)

Fc-gamma binding

Routine surface phenotyping

Phenotyping with in vitro stimulation and intracellular staining

Cell sorting

Cell separations

Biomarker/Metabolite Profiling

BCR/TCR Repertoire


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Please complete the following form to request services from the RBL Animal Models Unit:

https://app.smartsheet.com/b/form/21c0c1931115497db41fc0a0203a6b2e


IMPORTANT: Return to this form, check the box to indicate that you have submitted your request, and complete the remainder of this form.

Please complete the following form to request services from the RBL Immunology Unit:

https://app.smartsheet.com/b/form/747c8ba69705445d81dac97d4ab8d177


IMPORTANT: Return to this form, check the box to indicate that you have submitted your request, and complete the remainder of this form.

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Please describe the agent for which you require a safety assessment

When would you like to begin working with this agent?


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If YES, please upload below. If NO, please contact DCVC Program Management for support in developing a TPP.

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Please upload if appropriate.

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