Genomics and Microarray CoreSingle Cell Sample Submission Form

Genomics and Microarray Core

Single Cell Sample Submission Form

***Before you begin***

Please download the following Sample Information Template,

fill it out, and attach it to the end of this form:

Though very rare, it is possible that your samples could be lost due to instrument malfunction or human error. Please take precautions with precious/irreplaceable samples

Drop-off Instructions

TIMING for Single Cell Capture is CRITICAL to the success of your experiment and our schedule. We need you to provide a time that you will be delivering the cell/nuclei suspension to our facility. Preferably your delivery time will be between 10am to 2pm Monday through Thursday (Friday 10am -1pm). You may deliver cells/nuclei 30 minutes prior to this scheduled time or up to 1 hour after the scheduled time. Do not arrive after the 1 hour window as we WILL NOT capture your cells.

Please email both individuals below to schedule a time for sample drop off

Zhenghui Liu

Email: zhenghui.liu@cuanschutz.edu

Okyong Cho

Email: okyong.cho@cuanschutz.edu

Hours of operation for sample drop off:

Monday – Thursday, excluding University Holidays

10:00am to 2:00pm

Friday, excluding University Holidays

10:00am to 1:00pm

Location/Shipping Address:

Genomics and Microarray Core

University of Colorado Anschutz Medical Campus

12700 E.19th Ave

Bldg. RC-2, Room 9400

Aurora, CO 80045

Contact Information

PI Last Name*

PI First Name*

PI Email*

Post Doc/Student/Tech Name

Post Doc/Student/Tech Email

Preferred Contact*

Please select the preferred contact for this order

Post Doc/Student/Tech

Phone number

Please enter in the preferred contact phone number

Institution

Address

City

State

Zip code

Experiment and Sample Information

Species*

Select or enter value

Library Type*

Select or enter value

How many reads per cell do you want for Gene Expression (Standard: 50,000 reads/cell)?

V(D)J Library Type

Select or enter value

How many reads per cell do you want for V(D)J (Standard: 5,000 reads/cell)?

How many reads per cell do you want for ATAC (Standard: 25,000 reads/cell)?

How many reads per cell do you want for Gene Expression (Standard: 50,000 reads/cell)?

How many reads per cell do you want for ATAC (Standard: 25,000 reads/cell)?

Cell Surface Protein Label Type

Select or enter value

How many reads per cell do you want for Cell Surface Protein Label (Standard: 5,000 reads/cell)?

CellPlex Kit Type

Select or enter value

How many reads per cell do you want for CellPlex (Standard: 5,000 reads/cell)?

IMPORTANT DISCLOSURE: Customer should count cells after sorting via Hemocytometer and submit to core facility at appropriate concentration of 700-1100 cells/ul. Please follow 10X Genomics guidelines that were provided to you during the consultation meeting. The Genomics Core Facility cannot take responsibility for counting and diluting your cells. The 10X Chromium captures approximately 50-60% of input cells. Miscounting cells leads to inaccurate target cell number capture that directly affects sequencing. This error has the potential to render the data useless and it may not be recoverable if too many cells are captured.*

If there is a wetting failure, I agree the remaining sample will be used to repeat the capture.

I agree

Number of Samples (Per Capture Day)*

Select or enter value

Special Instructions (e.g. postpone sequencing until enough libraries for a flow cell lane)

Please enter in the email address of the person who will be receiving data via Server or FTP Account*

How would you like to receive your data?*

NOTE: Selecting the wrong option may delay the delivery of your data. If unsure, please ask your Bioinformatician about their preference.

Please enter your Globus ID (e.g. "shadete@access-ci.org")

Please attach any required material for your order.*

Sample information (Required)

Drag and drop files here or

Payment Information:

Are you a Gates Center Member?

Yes

Are you a Cancer Center Member?

Yes

Are you affiliated with the University of Colorado?*

Yes

7. Speedtype

Please enter speed type if affiliated with the University of Colorado

Sequencing Grant

Yes

Please specify the award recipient and award number

RBI Grant

Yes

Please specify the award recipient and award number

Payment method if not affiliated with the University of Colorado

Payment by check

Payment by credit card

Payment by wire transfer

Required signatures

Please have both the Principle Investigator (PI) that will be paying for the Sequencing Services and the Research/Technician/Student/Post-Doc below, acknowledging that all of the information provided on the form is correct. Signature of this form acknowledges that the PI and Technician/Student/Post-Doc have agreed to all sample submission, quality, quantity, project scheduling, and researcher financial responsibility requirements. Signature of this form authorizes the CU Genomics and Microarray Core to order all consumables necessary for the researcher’s sequencing project and confirms that the PI is financially responsible for items ordered for their project and all labor cost associated with the project.

Required Signature: Principle Investigator*

By typing your name into the space below you hereby agree to the above statement as the Principle Investigator. Please add your first/last name, and the date you are signing.

If you are signing on behalf of the Principle Investigator please note that they will receive a copy of this request.

Required Signature: Technician/Student/Post-Doc*

By typing your name into the space below you hereby agree to the above statement as the technician/student/post-doc. Please add your first/last name, and the date you are signing.

If you are the PI filling out this request please write N/A in the box below.

Data Sample and Retrieval

All data from sequencing run will be deleted from our servers 31 days after you receive notification that your data is ready to be downloaded from our server via email.

Please pick up constructed libraries no later than 60 days after data has been downloaded from our server. If you do not pick up your libraries they will be destroyed.

Send me a copy of my responses

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