Genomics and Microarray Core

Sequencing Sample Submission Form

***Before you begin***


Please download the following Sample Information Template,

fill it out, and attach it to the end of this form:


>>> SAMPLE INFORMATION TEMPLATE <<<


Though very rare, it is possible that your samples could be lost due to instrument malfunction or human error. Please take precautions with precious/irreplaceable samples

Contact Information

Preferred Contact*

Please select the preferred contact for this order

Please enter in the preferred contact phone number

Experiment and Sample Information

Note: All material submitted will be quality tested and quantitated. Any remaining material can be retrieved from the core by the customer after they receive their sequencing data. Any material not retrieved by the customer after 60 days will be discarded.

Library Information*
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IMPORTANT: Please pool your libraries into one tube. Your library concentration must be 10nM in 30uL. Concentration must be determined by Qubit.*
IMPORTANT DISCLOSURE: It is customer’s responsibility to ensure your library is compatible with the Illumina sequencing run option you choose below. We will do our best to get you at least 80% of your requested read depth, though there is no read number guarantee for customer-derived sequencing libraries. Please note that if barcode collision occurs due to your providing of wrong index sequences, you will be held liable and charged for the initial sequencing fee, the re-sequencing fee, and the re-sequencing fee of the other customers' samples. Please pool your libraries into one tube. Your library concentration must be 10nM in 30uL. Concentration must be determined by Qubit.*
4. Are custom primers required?
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If you would like to add PhiX to your NextSEQ 2000 order please indicate at what percentage, otherwise leave field blank.

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Consult your Bioinformatician to determine the sequencing depth necessary for you.


Standard Sequencing Depths:

  • Poly A Selected Total RNA-seq: "40M read pairs/80M total reads"
  • Ribo Depleted Total RNA-seq: "50M read pairs/100M total reads"
  • Low Input Ribo Depleted Total RNA-seq: "80M read pairs/160M total reads"
  • Small RNA-seq: "30M read pairs/60M total reads"
  • ChIP/Cut&Run-seq: "10M read pairs/20M total reads"

Consult your Bioinformatician to determine the sequencing depth necessary for you.


Standard Sequencing Depths:

  • Genomic DNA-seq (WGS): "30X - 50X Coverage"
  • Exome-seq (WES): "100X - 400X Coverage:
How would you like to receive your data*

NOTE: Selecting the wrong option may delay the delivery of your data. If unsure, please ask your Bioinformatician about their preference.

Sample information (Required)

Tapestation/Bioanalyzer Results (optional)

Qubit/Nanodrop Results (optional)

Drag and drop files here or

Payment Information:

Are you a Gates Center Member?
Are you a Cancer Center Member?
Are you affiliated with the University of Colorado?*

Please enter speed type if affiliated with the University of Colorado

Sequencing Pilot Award
RBI Pilot Award
Payment method if not affiliated with the University of Colorado

Email or call to schedule a time for sample drop off


Nicole Manning

Email: Nicole.Manning@CUAnschutz.edu

Phone: (303) 724-6050


Hours of operation for sample drop off:

Monday – Friday, excluding University Holidays

9:00am to 3:00pm


Location/ Shipping Address:

Genomics and Microarray Core

University of Colorado Anschutz Medical Campus

12700 E.19th Ave

Bldg. RC-2, Room 9400

Aurora, CO 80045

Required signatures

Please have both the Principle Investigator (PI) that will be paying for the Sequencing Services and the Research/Technician/Student/Post-Doc below, acknowledging that all of the information provided on the form is correct. Signature of this form acknowledges that the PI and Technician/Student/Post-Doc have agreed to all sample submission, quality, quantity, project scheduling, and researcher financial responsibility requirements. Signature of this form authorizes the CU Genomics and Microarray Core to order all consumables necessary for the researcher’s sequencing project and confirms that the PI is financially responsible for items ordered for their project and all labor cost associated with the project.

By typing your name into the space below you hereby agree to the above statement as the Principle Investigator. Please add your first/last name, and the date you are signing.


If you are signing on behalf of the Principle Investigator please note that they will receive a copy of this request.

By typing your name into the space below you hereby agree to the above statement as the technician/student/post-doc. Please add your first/last name, and the date you are signing.


If you are the PI filling out this request please write N/A in the box below.

Data Sample and Retrieval

All data from sequencing run will be deleted from our servers 31 days after you receive notification that your data is ready to be downloaded from our server via email.

Please pick up original DNA / RNA submitted along with constructed libraries no later than 60 days after data has been downloaded from our server. If you do not pick up your samples and libraries they will be destroyed.

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