Sort Form - Link Hogthrob

Please answer the questions below to allow your sort to go smoothly. Thank you!

Have you pressed the Complete Booking button in PPMS?

Please enter your PPMS user name

Date of Sort

Please enter the Date you booked in PPMS

Nozzle

Downstream Processing

Will these sorted samples be sent to the Genomics Research Center (GRC) for downstream processing/analysis?

BSL Level

Sample Origin

Sample Type

Sample and Population Info

Has this project been reviewed by the IBC?

Are these cells infected?

Are these cells from an animal that has been exposed to a human pathogen or infectious agent?

Number of Samples to Sort

Number of Populations

Purity Check Post Sort Requested

Temperature Requirements

Sample and Collection Temperature

Must be between 4C and 37C and both temperatures must be the same

Collection Vessel

Sort Precision Mode

Is this a new panel?

Have you created or run this panel on any of the following instruments?

How many fluorochromes will you be using?

Enter any additional information you would like to give us below

For example, other sample types, multiple collection media, aseptic sort needs, custom plates, or temperature settings

Please keep in mind the following as you prepare for your sort:

- Bring samples in locking secondary containment with biohazard label

- Bring proper controls

- Concentration is nozzle dependent check the link below for specifics

- Know your sample volume

- How many cells will you need to collect to set up gates?

- How many events do you need for analysis?

- Type/size of collection vessel

- How many cells do you want/can we collect per tube or well?

- What fluorochromes are you using?

- Special needs: Please discuss with us

- Further details and assistance can be found on our website, https://www.urmc.rochester.edu/research/flow-core/services/instruments/sorting.aspx

Send me a copy of my responses