Sort Form - Agnes Stonewick

Sort Form - Agnes Stonewick

Please answer the questions below to allow your sort to go smoothly. Thank you!

Have you pressed the Complete Booking button in PPMS?

Please enter your PPMS user name*

Date of Sort*

Please enter the Date you booked in PPMS

Nozzle

Select

Downstream Processing

Will these sorted samples be sent to the Genomics Research Center (GRC) for downstream processing/analysis?

BSL Level

Select

Sample Origin

Select or enter value

Sample Origin - Other

Please enter the origin of the sample

Sample Type

Select or enter value

Cell Line Name

Sample and Population Info

Has this project been reviewed by the IBC?

GNT Note

GNT Number (G Form)

Are these cells infected?

What is the infecting organism?

Are these cells from an animal that has been exposed to a human pathogen or infectious agent?

What is the pathogen or agent?

Number of Samples to Sort

Number of Populations

Purity Check Post Sort Requested

Post Sort Note

Temperature Requirements

Sample Temperature

Select

Collection Temperature

Select

Collection Vessel

Select

Sort Precision Mode

Select

Is this a new panel?

Have you run this panel on this instrument before?

Do you have a template?

Enter Template Name

How many fluorochromes will you be using?

List of Flurochromes

Please list one per line

Enter any additional information you would like to give us below

For example, other sample types, multiple collection media, aseptic sort needs, custom plates, or temperature settings

Please keep in mind the following as you prepare for your sort:

- Bring samples in locking secondary containment with biohazard label

- Bring proper controls

- Concentration is nozzle dependent check the link below for specifics

- Know your sample volume

- How many cells will you need to collect to set up gates?

- How many events do you need for analysis?

- Type/size of collection vessel

- How many cells do you want/can we collect per tube or well?

- What fluorochromes are you using?

- Special needs: Please discuss with us

- Further details and assistance can be found on our website, https://www.urmc.rochester.edu/research/flow-core/services/instruments/sorting.aspx

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